Journal

ACE Research Journal of Microbiology and Biotechnology

  • ISSN Print: 2520-3975, ISSN Online:
  • Start Year: 2021
  • Volume: 4 (2024), Issue: 1

Evaluation of Barley Genotypes Under Salinity Stress Conditions and their Molecular Characterization Using ISSR Markers

Author: Elaf muner Abd Al‐Kadhim Al‐Khafaji, Jehan Ahmed Aflook and Amna Ahmed Abbas
Page: 6-10
Published online: 05 Aug 2024

https://doi.org/acerjmb.2024.6.10

Abstract:

The experiment was carried out in College of Education for Girls / Department of biology / University of Kufa 2023. The two genotypes of barley H9 and H20 were used derived from breeding and improvement programs in the fields of the Iraqi Atomic Energy Organization previously. The two sensitive varieties, Furat 6 and Furat 7, were used for the purpose of comparison. The increase in salinity at the 16 Ds. m‐1 salt level led to a significant decrease in the germination rate for the two genotypes and the two local varieties used in the experiment, but there is a clear difference in the degree of effect, as it was The germination rate for the two genotypes H9 is 70% and H20 is 61%, while the germination rate was 24% in the Furat6 variety and decreased at its lowest levels to reach 15.5% in the Furat7 variety, which is considered the most affected by salinity. The results showed that the primers ISSR‐UBC 818 and ISSR‐UBC 840, ISSR‐UBC 812 and ISSR‐UBC 842, which were able to show a number of general bands in all the studied varieties, but failed to show distinctive bands.