Author:
Ahmed Al Obaidi, Aqeel A. Alsadawi and Zhraa Hameed Oda Alquraishi
Page: 18-22
Published online: 11 May 2024
https://doi.org/acerjmb.2023.18.22
From November 2022 to February 2023, a total of 50 clinical specimens were taken from individuals with blood infections. Patients who visited AL‐Sadder Medical City and AL‐Hakem General Hospital during the study time provided these specimens. All samples were grown on blood agar and MacConkey agar plates and they were all then incubated for 18‐24 hrs at 37EC under aerobic conditions. Among a sample of 50 patients, an equal number of 25 (50%) were identified as female and 25 (50%) as male. Various morphological, physiological and biochemical examinations were conducted in order to ascertain the identification of bacterial isolates. The findings of the study indicate that A. baumannii accounted for 8 isolates 16% of the total isolates, whereas the remaining isolates were identified as E. coli and P. aueroginosa. The study involved a set of 8 isolates of A. baumannii, which were identified based on their morphological, cultural and biochemical characteristics. The final confirmation of their identification was carried out using the Vitek‐2 compact system, which revealed that only 6 (75%) of the isolates were indeed diagnosed as A. baumannii. The research examined the genes responsible for encoding virulence factors in A. baumannii, which are crucial for its pathogenicity, including pap and FimA. The aforementioned genes play a significant role in the processes of invasion and adhesion. The results of the research demonstrated that A. baumannii virulence factors are crucial to the pathogenesis of the bacteria.