TY  - JOUR
T1  - Molecular Detection of Enterotoxogenic Bacterial Toxins Isolated from Human Stool and Beef
AU - Ramadhan, Mukhallad
        AU - Aljeboori, Khalil
        
JO  - ACE Journal of Research Studies in Biosciences
VL  - 5
IS  - 1
SP  - 30
EP  - 37
PY  - 2025
DA  - 2001/08/19
SN  - 2520-3916
DO  - 10.36478/acejrsb.2025.30.37
UR  - https://theacepublications.com/article-detail.php?art=270-ACE-JRSB
KW  - Beef samples
KW  - human stool samples
KW  - ETEC
KW  - Non‐O157
AB  - Characterization of non‐O157 enterotoxogenic Escherichia coli,
particularly the important strains that cause severe illnesses has
lagged considerably behind that of strain O157. In this study
specification of enterotoxogenic <i>E coli</i> from other non‐ O157 was
accomplished by PCR by using primers for wbdI (O antigen‐ encoding
gene cluster). Virulence of the detected strains was determined by
detection of (<i>stx1, stx2, eae and hlyA</i>) virulence factors genes by
multiplex PCR technique in order to determine the aggressiveness
of the strains. Later on the wbdI gene was sequenced in order to confirm
the PCR results. Results showed that the number of ETEC isolates
confirmed by PCR was 8; among which 6 from human diarrheal cases
and 2 from beef samples. Regarding human isolates, molecular
detection of virulence factors genes showed that two of human stool
isolates had the 4 virulence genes and 3 had three genes. Two strains
out of the three contained (<i>stx1, eae, hlyA</i>) and one contain (<i>stx1, stx2,
eae</i>). One of human stool isolates contains two virulence genes (<i>stx1,
stx2</i>). In the beef isolates the results showed that one of them contains
(<i>stx1, stx2</i>) and the other contains (<i>eae, hlyA</i>). The present study
concludes that the ETEC is found in both beef samples and human stool,
this may indicate the risk of transmission from animal to the human
through the contaminated meat.
ER  -